THE EFFECTS FERMENTED NATURAL PLANT GROWTH REGULATOR ON PRANCAK 95 TOBACCO (NICOTIANA TABACUM L. VAR PRANCAK 95) ACCLIMATIZATION

Authors

  • Sepdian Luri Asmono Politeknik Negeri Jember
  • I Harlianingtyas
  • Dhanang Eka Putra

Abstract

Prancak 95 tobacco has the potential to be developed in Indonesia because it has a distinctive aroma and a nicotine content of 2.13%. The quality of this tobacco raw material must be improved and started from the use of quality seeds through in vitro culture techniques. However, several things that must be considered are the acclimatization stages. One of the efforts to increase the growth of seedlings during the acclimation can be done by providing natural plant growth regulators (PGR) from extracts of organic materials such as coconut water, young corn, sprouts, and banana weevil that has been fermented in advance using EM-4 bioactivators . Based on this, the purpose of this study was to determine the optimal concentration of natural growth regulators in the acclimatization of Prancak -95 tobacco. This study uses a non factorial randomized block design with four treatments concentrated solution of natural growth regulators, which include: Control (no treatment), 10; 25; and 50 ml/L. The observed variables included the number of leaves, leaf area, plant height, and root length. Data analysis using Analysis of Variance  (ANOVA) Test and continued with Duncan's Multiple Range Test (DMRT) Test at 95% confidence level to find out the best treatment. Based on the results of research that has been carried out for 60 days of observation, it can be concluded that the application of natural PGR is able to spur the growth of seedlings during the acclimatization period. Spraying application at a concentration of 10 ml / L can increase the number of leaves, leaf area, number of roots and height of Prancak 95 tobacco seedlings.

References

N. Kumar and M. Reddy, “In vitro Plant Propagation: A Review,†J. For. Environ. Sci., vol. 27, 2011.

J. Pospóšilová, I. Tichá, P. KadleÄek, D. Haisel, and Å . Plzáková, “Acclimatization of micropropagated plants to ex vitro conditions,†Biol. Plant., vol. 42, no. 4, pp. 481–497, 1999.

S. Anwarudin, “Uji Keberadaan Zat Pengatur Tumbuh (ZPT) Organik Auksin dari Tauge dan Bonggol Pisang yang telah Difermentasi Menggunakan MOL, EM-4, dan PGPR dengan MetodeHPLC,†Universitas Islam Negeri Sunan Kalijaga Yogyakarta, 2017.

J. W. H. Yong, L. Ge, Y. F. Ng, and S. N. Tan, “The chemical composition and biologi cal properties of coconut (Cocos nucifera L.) water,†Molecules, vol. 14, no. 12, pp. 5144–5164, 2009.

W. Admaja, H. Sulistyowati, and S. Sarbino, “Pengaruh Campuran Hormon Organik dan Pupuk Organik Cair terhadap Peningkatan Daya Tumbuh Bibit Stum Mata Tidur Tanaman Karet,†Perkeb. dan Lahan Trop., vol. 4, no. 2, pp. 18–21, 2015.

U. Kaffi, “Uji Efektifitas Pertumbuhan Vegetatif Bunga Nusa Indah (Mussaenda Pubescens) Terhadap Pemberian ZPT Organik Jagung Muda Pada Berbagai Sumber Setek,†Agrovital, vol. 2, no. 2, 2017.

F. Kurniati, T. Sudartini, and D. Hidayat, “Aplikasi berbagai bahan ZPT alami untuk meningkatkan pertumbuhan bibit kemiri sunan (Reutealis trisperma (Blanco) Airy Shaw),†J. Agro, vol. 4, no. 1, pp. 40–49, 2017.

P. J. Davies, “The plant hormones: their nature, occurrence, and functions,†in Plant hormones, Springer, 2010, pp. 1–15.

J.-M. Davière and P. Achard, “Organ communication: Cytokinins on the move,†Nat. plants, vol.3, no. 8, p. 17116, 2017.

J. Skalák et al., “Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis,†Plant J., vol. 97, no. 5, pp. 805–824, 2019.

E. Tanimoto and K. Hirano, “Role of Gibberellins in Root Growth,†in Plant Roots: The Hidden Half, Fourth Edition, 2013, pp. 11–13.

E. Tanimoto, “Regulation of Root Growth by Plant Hormones—Roles for Auxin and Gibberellin,†CRC. Crit. Rev. Plant Sci., vol. 24, no. 4, pp. 249–265, 2005.

K. Takahashi and T. Kinoshita, “The regulation of plant cell expansion: auxin-induced turgordriven cell elongation,†Mol. cell Biol. growth Differ. plant cells, pp. 156–173, 2016.

Published

27-12-2019