ENDOGENOUS HORMONES AT THE BEGINNING OF LEAF GROWTH AFTER VERNALIZATION OF GARLIC BULB (ALLIUM SATIVUM L.) IN INDONESIAN LOCAL VARIETIES

Abstract

Flowering of garlic in tropical regions such as Indonesia has never been found, so that generational multiplication cannot be done yet. Induction of garlic flowering can be done by vernalizing the seed tubers to trigger flowering. This study aims to determine the hormone status  of  IAA,  GA 3 ,  at  the  time  of  initial  growth  of  leaf  tissue,  after  going  through vernalization. Bulbs of Indonesian local varieties of Lumbu Kuning and Tawangmanu varieties were vernalized for 4 weeks, 8 weeks, and without vernalization treatment as a control. The contents of IAA and GA hormones in the leaf tissue of garlic plants aged 2 weeks were analyzed using method  high performance liquid chromatography (HPLC). The results showed that the Lumbu Hijau variety with 4 weeks vernalization contained IAA of 1615,595 ng / g; GA 3 is 7.297 ng / g. At 8 weeks vernalization the IAA content is 2169,475 ng / g; GA 3 of 9,757 ng / g; In the Tawangmanu variety with 4 weeks vernalization containing IAA of 1459,168 ng / g; GA 3 is 6.617 µg / g. Whereas for 8 weeks vernalization of IAA content of 2007,195 ng / g; GA 3 is 8.315 ng / g.