KARAKTERISASI BAKTERI PENGHASIL EKSOPOLISAKARIDA ASAL RHIZOSFER TANAMAN KENTANG SEBAGAI PROMOTOR PERTUMBUHAN TANAMAN

Mu’minah Mu’minah, Junyah Leli Isnaeni, Baso Darwisah

Abstract


Utilization of Exopolisakarida Bacteria as adhesive agent and binder soil structure so that the structure of the soil becomes steady. EPS bacteria play a role in stabilizing soil aggregates through augmentation techniques in potato plant rhizosphere.This study aims to characterize the bacteria producing eksopolisakarida (EPS) obtained from the rizosphere of highland potato plants which are focussed as promoters of plant growth. There are 3 sample sampling locations that are distinguished by the height of the sea level: 1200, 1500 and 1800 m above sea level in in Malino, South Sulawesi. Soil samples were diluted and cultured on ATCC medium No. 14 and the specific medium of exopolisakarida-producing bacteria Mac Concay medium. After testing there were 15 EPS bacterial isolates that had the potential to produce exopolysaccharides and all the bacteria were grouped in gram-negative bacteria. Isolates P3T (63) yielded an IAA ( Indole Acetic Acid) value of 21.14 ppm and as a phosphate solvent of 20, 59 ppm. While Isolate P3T (50) has the ability to fix Nitrogen of 1, 42 ppm, and produce EPS of 1,07 mg/mg protein with the best carbon source for producing exopolysaccharides is Sucrose.

 

Keywords : Exopolysaccharide, Promoters, IAA, Phosphate solvent, Nitrogen Fixation


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DOI: https://doi.org/10.25047/jii.v19i2.1500

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