RESISTENSI ANTIBIOTIKA Bifidobacterium PADA KEFIR dan YOGURT

Authors

  • Titik Budiati
  • Wahyu Suryaningsih

Abstract

Sebanyak 12 isolat Bifidobacterium diisolasi dari sampel kefir dan yogurt yang diperoleh dari pasar lokal. Resistensi Bifidobacterium yang diisolasi dari kefir dan yogurt mempunyai sifat resistensi terhadap terhadap Ceftazidime (Caz, 100 %), Ceftriaxone (Cro, 100%), Lincomycin (Li, 100%), Rifampicin (Rd, 100%), Sulphamethoxazole-Trimethoprim (Sxt, 50%), Tobramycin (Tob, 100%). Antibiogram yang paling banyak dijumpai pada Bifidobacterium adalah CazCroLiRdSxtTob (n=5) dengan. MAR index yang bervariasi dari 0.83 sampai 1. Tingginya MAR index menunjukkan semakin banyak paparan kontaminasi antibiotika pada bahan pembuatan yogurt dan kefir.

References

A. Rosander, E. Connolly, dan S. Roos, Removal of antibiotic resistance gene-carrying plasmids from Lactobacillus reuteri ATCC 55730 and characterization of the resulting daughter strain, L. reuteri DSM 17938. Applied and environmental microbiology, 2008. 74(19), 6032-6040.

V. Vankerckhoven, G. Huys, M. Vancanneyt, C. Vael, I. Klare, M.-B Romond, J.M. Entenza, P. Moreillon, R.D. Wind, J. Knol, E. Wiertz, B. Pot, E.E. Vaughan, G. Kahlmeter, H. Goossens, Biosafety assessment of probiotics used for human consumption: recommendations from the EUPROSAFE project. Trends Food Sci. Technol. 2008. 19:102–114.

M. Ventura, C. Canchaya, A. Tauch, G. Chandra, K. Chater, G.F. Fitzgerald, dan D. Van Sinderen. Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum. Microbiol. Mol. Biol. Rev. 2007.71:495-548.

R. Lamendella, J.W. Santo Domingo, C. Kelty, dan D.B. Oerther, Bifidobacteria in feces and environmental waters. Appl. Environ. Microbiol. 2008.74:575-584

Makino, Hiroshi, Akira Kushiro, Eiji Ishikawa, Hiroyuki Kubota, Agata Gawad, Takafumi Sakai, Kenji Oishi et al. "Mother-toinfant transmission of intestinal bifidobacterial strains has an impact on the early development of vaginally delivered infant's microbiota." PloS one 8, 2013. no. 11: e78331.

B.L. Maidak, J.R. Cole, T.G. Lilburn, C.T. Parker, P.R. Saxman, R.J. Farris, G.M. Garrity, G.J. Olsen, T.M. Schmidt, dan J.M. Tiedje, The RDP-II (Ribosomal Database Project). Nucleic Acids Res. 2001. 29:173-174.

M.L. Marco, S. Pavan, dan M. Kleerebezem, Towards understanding molecular modes of probiotic action. Curr. Opin. Biotechnol. 2006. 17:204-210

A. Sapkota, A.R. Sapkota, M. Kucharski, J. Burke, S. McKenzie, P. Walker, dan R. Lawrence, Aquaculture practices and potential human health risks: current knowledge and future priorities. Environment International 2008. 34, 1215-1226.

P.H. Serrano. Responsible use of antibiotics in aquaculture. FAO Fisheries Technical Paper. No. 469, Food and Agriculture Organization of The United Nation, Rome, Italy, pp. 1-10. 2005

K. Kümmerer, K., dan A. Henninger. Promoting resistance by the emission of antibiotics from hospitals and households into effluents. Clinical Microbiology and Infections, 2003. 1203–1214.

J.L. Martinez. Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental pollution, 2009. 157: 2893-2902.

S.B. Levy, dan B. Marshall. Antibacterial resistance worldwide: causes, challenges and responses. Nature Medicine, 2004. 10 (Suppl): S122–S129

A.M. Sefton. Mechanisms of antimicrobial resistance. Drugs, 2002. 62: 557–566.

A.J. Alanis. Resistance to antibiotics: are we in the post-antibiotic era? Archives of medical research, 2005, 36: 697–705.

G.A. Jacoby dan L.S. Munoz-Price. The new β-lactamases. The New England Journal of Medicine, 2005. 352: 380–391.

CLSI. 2010. “Performance Standards for Antimicrobial Disk Susceptibility Tests, Informal Supplementâ€. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, 2010.

P.H. Krumperman. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods.Applied and Environmental Microbiology, 1983. 46(1), 165-170.

M.R. D'Aimmo, M. Modesto, B. Biavati, Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products. International journal of food microbiology, 2007, 115(1), 35-42

W.P. Charteris, P.M. Kelly, L. Morelli, L., dan J.K. Collins.Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. Journal of applied microbiology, 1998,84(5), 759-768.

A.M. Yazid, A.M. Ali, M. Shuhaimi, V. Kalaivaani, M.Y. Rokiah, A. Reezal. Antimicrobial susceptibility of bifidobacteria. Letters in applied microbiology, 2002. 31(1), 57-62.

L. Masco, K. Van Hoorde, E. De Brandt, J. Swings, dan G. Huys, Antimicrobial susceptibility of Bifidobacterium strains from humans, animals and probiotic products. Journal of Antimicrobial Chemotherapy, 2006. 58(1), 85-94.

M.A. Webber, V. Ricci, R. Whitehead, M. Patel, M. Fookes, A.Ivens, dan L.J.V. Piddock. "Clinically relevant mutant DNA gyrase alters supercoiling, changes the transcriptome, and confers multidrug resistance." MBio 4, 2013. no. 4: e00273-13.

C. Pál, B. Papp, M.J. Lercher.. Adaptive evolution of bacterial metabolic networks by horizontal gene transfer. Nature genetics, 2005. 37(12), 1372-1375.

O.O. Osundiya, R.O. Oladele, O. O.Oduyebo. (2013). Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University TeachingbHospital. African Journal of Clinical and ExperimentalbMicrobiology, 2013. 14(3), 164-168.

Published

05-12-2016