Induksi Kalus Dari Beberapa Kultivar Tanaman Kentang (Solanum tuberosum L.) Dataran Medium Secara In Vitro menggunakan Variasi Konsentrasi 2,4-D

Abstract

This study aims to determine the concentration of 2,4-D to grow a callus on several potato medium cultivars such as DTO-28 and Desiree. This study uses a completely randomized design factorial. Factors to be tested is the concentration of 2,4-D: 4.53; 9.05;13.58; 18.10 μM and Potato Cultivar: DTO-28 and Desiree. There are a total of 8 treatments with 5 replications. Parameters measured include the time appeared callus, callus fresh weight, percentage of callus formation, as well as the morphology of callus kualitif parameters, such as texture, color, type of callus, the number of shoots and roots. Data observation of callus morphology presented descriptively, while other data were analyzed by analysis of variance (ANOVA). To know the difference between the treatment performed DMRT at 5% level. The results of the study, demonstrate that all explants of cultivar Desiree and DTO-28 capable to forming callus, with watery crumb texture and average white and yellowish green. DTO-28 cultivars are more responsive to 2,4-D because emerge faster callus which 5 DAP. Both cultivars tested were able to produce callus heaviest at 18.10 μM concentrations of 2,4-D. From these results it can be concluded that the concentration of 18.10 μM concentrations of 2,4-D is the best in spurring the growth of callus potatoes DTO-28 and Desiree.