PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY FROM FRUIT OF KULIM (SCORODOCARPUS BORNEENSIS BECC.)
Abstract
This research was carried out to determine the phytochemical and antioxidant activity from the fruit of Kulim (Scorodocarpus borneensis Becc.). The partition of extract was prepared by fractionation using gradient elution with hexane, ethyl acetate, ethanol, methanol, and 70% methanol respectively and stirred for 24 hours. The crude fraction then concentrated using rotary evaporator at 40℃. Qualitative determination of fruit fractions by phytochemical screening were tested for the presence of flavonoids, alkaloids, tannins, phenols and terpenoids, while quantitative determination of total phenol and antioxidant activity by DPPH free radical scavenging activity was carried out using colorimetric methods. The yields of crude fraction were obtained 0,47% (hexane), 0,09% (ethyl acetate), 0,30% (ethanol), 1,23% (methanol) and 1,89% (70% methanol). Phytochemical screening of S. borneesis fruit crude fractions revealed the presence of all tested variable on ethanol, methanol and 70% methanol solvent. The highest total phenolic content and antioxidant activity of Kulim fruits was obtained from fractionation with methanol solvent, that is 927,36 mgGAE/g and 14,54ppm respectively.This research was carried out to determine the phytochemical and antioxidantactivity from the fruit of Kulim (Scorodocarpus borneensis Becc.). The partition of extract wasprepared by fractionation using gradient elution with hexane, ethyl acetate, ethanol, methanol,and 70% methanol respectively and stirred for 24 hours. The crude fraction then concentratedusing rotary evaporator at 40℃. Qualitative determination of fruit fractions by phytochemicalscreening were tested for the presence of flavonoids, alkaloids, tannins, phenols andterpenoids, while quantitative determination of total phenol and antioxidant activity by DPPHfree radical scavenging activity was carried out using colorimetric methods. The yields ofcrude fraction were obtained 0,47% (hexane), 0,09% (ethyl acetate), 0,30% (ethanol), 1,23%(methanol) and 1,89% (70% methanol). Phytochemical screening of S. borneesis fruit crudefractions revealed the presence of all tested variable on ethanol, methanol and 70% methanolsolvent. The highest total phenolic content and antioxidant activity of Kulim fruits wasobtained from fractionation with methanol solvent, that is 927,36 mgGAE/g and 14,54ppmrespectively.References
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