Effect of Pineapple Extract Concentration and Soaking Duration on Germination and Vigor of Seedless Watermelon (Citrullus lanatus L.) Seeds
DOI:
https://doi.org/10.25047/tefa.v3i2.6872Abstract
Seedless watermelon (Citrullus lanatus L.) seeds commonly exhibit physical dormancy due to a hard and impermeable seed coat that restricts water uptake and delays germination. Pineapple extract containing the proteolytic enzyme bromelain offers a natural alternative to mechanical cracking by facilitating seed coat softening. This study evaluated the effects of pineapple extract concentration and soaking duration on dormancy breaking and early seed performance of seedless watermelon. A factorial Completely Randomized Design was applied using three extract concentrations (25%, 50%, and 75%) and three soaking durations (5, 10, and 15 minutes). Germination percentage, radicle emergence, and vigor index were measured. The results showed that extract concentration and soaking duration significantly affected germination percentage and vigor index, while their interaction significantly influenced radicle emergence. The highest performance was consistently achieved at a 25% extract concentration with 5 minutes of soaking, resulting in 64.22% germination, 70.67% radicle emergence, and 53.56% vigor index. These findings indicate that moderate enzymatic treatment enhances dormancy breaking by promoting protein hydrolysis and improving early metabolic activation. The novelty of this study lies in identifying the optimal interaction between pineapple extract concentration and soaking duration for improving multiple physiological indicators of seed performance. Pineapple extract can therefore be considered a practical, natural, and environmentally friendly alternative for improving dormancy breaking and early seed performance in seedless watermelon.
References
[1] Awasthi, P. (2023). Review on dormancy, causes, uses, and measures of overcoming it. Science Heritage Journal (GWS), 7(2), 57–60. https://doi.org/10.26480/gws.02.2023.57.60
[2] Baskin, C. C., & Baskin, J. M. (2014). Seeds: Ecology, biogeography, and evolution of dormancy and germination (2nd ed.). Academic Press.
[3] Dzulqaidah, I., Zanuba, R. B., Alwi, A. S. F., & Muliasari, H. (2021). Ekstraksi dan uji aktivitas enzim bromelin kasar dari buah nanas. Journal of Agritechnology and Food Processing, 1, 80–87.
[4] Eshkab, I. A., & Harris, P. J. C. (2020). Seed priming: Factors affecting efficacy. International Review of Basic and Applied Sciences (IRBAS), 8, 31–52. https://www.irbas.academyirmbr.com/
[5] Finch-Savage, W. E., & Footitt, S. (2017). Seed dormancy cycling and the regulation of dormancy mechanisms to time germination in variable field environments. Journal of Experimental Botany, 68(4), 843–856. https://doi.org/10.1093/jxb/erw477
[6] Gao, L., & Shi, H. (2024). Advancements in environmental factors regulating plant seed germination. Journal of Capital Normal University (Natural Science Edition), 45(4), 48–57. https://doi.org/10.19789/j.1004-9398.2024.04.007
[7] Henggu, K. U., & Nurdiansyah, Y. (2022). Review dari metabolisme karbohidrat, lipid, protein, dan asam nukleat. Jurnal Quimica, 3(2). https://doi.org/10.33059/jq.v3i2.5688
[8] Kumaunang, M., & Kamu, V. (2011). Aktivitas enzim bromelin dari ekstrak kulit nenas (Ananas comosus). Jurnal Ilmiah Sains, 11(2).
[9] Liu, S., Wang, W., Lu, H., Shu, Q., Zhang, Y., & Chen, Q. (2022). New perspectives on physiological, biochemical and bioactive components during germination of edible seeds: A review. Trends in Food Science & Technology, 123, 187–197. https://doi.org/10.1016/j.tifs.2022.02.029
[10] Longo, C., Holness, S., De Angelis, V., Lepri, A., Occhigrossi, S., Ruta, V., Vittorioso, P., & Paola, V. (2020). From the outside to the inside: New insights on the main factors that guide seed dormancy and germination. Genes, 12(1), 52. https://doi.org/10.3390/genes12010052
[11] Marditasari, M., Anggraeni, I., & Novitasari, E. (2015). Aplikasi seed priming menggunakan enzim bromelin dari limbah kulit nanas (Ananas comosus L.) dengan lama perendaman yang berbeda terhadap perkecambahan benih gambas (Luffa acutangula L.).
https://doi.org/10.13140/RG.2.2.13482.24002
[12] Nathania, D. S., & Bratadiredja, M. A. (2019). Isolasi dan uji stabilitas enzim bromelin dari nanas (Ananas comosus L.). Farmaka, 16(1). https://doi.org/10.24198/jf.v16i1.17508
[13] Nugrahini, T. (2015). Viabilitas dan pertumbuhan benih semangka non biji (Citrullus vulgaris Schard.) terhadap pengaruh suhu dan pemecahan kulit luar. Jurnal AGRIFOR, 14(1).
[14] Nurnaningsih, H., & Laela, D. S. (2022). Efektivitas daya antibakteri berbagai konsentrasi enzim bromelain dari ekstrak buah nanas (Ananas comosus [L.] Merr.) terhadap Streptococcus mutans secara in vitro. Padjadjaran Journal of Dental Researchers and Students, 6(2), 75–82. https://doi.org/10.24198/pjdrs.v6i1.38211
[15] Paparella, S., Araújo, S. S., Rossi, G., Wijayasinghe, M., Carbonera, D., & Balestrazzi, A. (2015). Seed priming: State of the art and new perspectives. Plant Cell Reports, 34, 1281–1293. https://doi.org/10.1007/s00299-015-1784-y
[16] Pérez, L., Acosta, Y., Nápoles, L., Linares, C., Sershen, & Lorenzo, J. C. (2021). Pineapple stem-derived bromelain-based priming improves pepper seed protein reserve mobilization, germination, emergence, and plant growth. Physiology and Molecular Biology of Plants, 27, 1651–1657. https://doi.org/10.1007/s12298-021-01038-7
[17] Pérez-Gómez, L., Acosta Fernández, Y., Báez Pérez, E., Linares Rivero, C., Carvajal Ortiz, C., Naidoo, S., & Pérez Martínez, A. (2022). Bromelain crude extract to improve the emergence and growth of pepper seedlings. Revista de Ciencias Agrícolas. https://doi.org/10.22267/rcia.202239E.195
[18] Rajiman, & Megawati, S. (2022). Viabilitas dan vigor berbagai varietas true shallot seed dengan perendaman jenis zat pengatur tumbuh (ZPT) alami. AGRITECH, 24(2).
[19] Rowan, A. D. (2013). Stem bromelain. In Handbook of proteolytic enzymes (3rd ed.). Academic Press.
[20] Rukmansyah, A., & Putri, I. M. (2025). Potensi nanas (Ananas comosus) sebagai bahan pangan fungsional kaya bromelain [The potential of pineapple (Ananas comosus) as a functional food rich in bromelain]. JUTHAP (Jurnal Teknologi Hasil Pertanian), 2(2), 65–71.
[21] Saputra, B., Kurniastuti, T., & Puspitorini, P. (2017). Pengaruh kombinasi skarifikasi dan perendaman auksin terhadap viabilitas benih dan pertumbuhan awal semangka non biji (Citrullus vulgaris Schard. L.). VIABEL: Jurnal Ilmu-Ilmu Pertanian, 11(2), 1–8.
https://doi.org/10.35457/viabel.v11i2.264
[22] Srilaba, N., Purba, J. H., & Arsana, I. K. N. (2018). Pengaruh lama perendaman dan konsentrasi atonik terhadap perkecambahan benih jati (Tectona grandis L.). Agro Bali (Agricultural Journal), 1(2), 108–119.
[23] Wahab, F. A., Kandowangko, N. Y., & Ahmad, J. (2025). Studi pematahan dormansi dalam meningkatkan daya kecambah benih padi (Oryza sativa L.) varietas Mekongga dengan perlakuan GA₃. Tumbuhan: Publikasi Ilmu Sosiologi Pertanian dan Ilmu Kehutanan, 2(1). https://doi.org/10.62951/tumbuhan.v2i1.173
[24] Wazeer, H., Gaonkar, S. S., Doria, E., Pagano, A., Balestrazzi, A., & Macovei, A. (2024). Plant-based biostimulants for seeds in the context of circular economy and sustainability. Plants, 13(7), 1004. https://doi.org/10.3390/plants13071004
[25] Zhao, M., Zhang, H., Yan, H., Qiu, L., & Baskin, C. C. (2018). Mobilization and role of starch, protein, and fat reserves during seed germination of six wild grassland species. Frontiers in Plant Science, 9, 234. https://doi.org/10.3389/fpls.2018.00234
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