Filogenetik Kutu Penghisap Darah (Haematopinus sp.) pada Beberapa Jenis Sapi berdasarkan Gen 18S rRNA

Authors

  • Aan Awaludin Politeknik Negeri Jember http://orcid.org/0000-0003-2491-3931
  • Yudhi Ratna Nugraheni Universitas Gadjah Mada
  • Mira Andriani Politeknik Negeri Jember
  • Niswatin Hasanah Politeknik Negeri Jember
  • Agus Hadi Prayitno Politeknik Negeri Jember

DOI:

https://doi.org/10.25047/jipt.v3i2.1918

Keywords:

DNA, Phylogenetic, Haematopinus sp., Louse, Cattle

Abstract

The purpose of this study was to determine the phylogenetic relationship of Haematopinus sp. which became parasites in several types of cattle, namely Simmental, Limousin, PO (Peranakan Ongole), and FH (Friesian Holstein) from Jember Regency and Simmental and PO cattle from Karanganyar Regency. The method used was to isolate DNA from 6 samples of Haematopinus sp. and amplified using 18S rRNA universal primers with 18S (5'-TCATTACGAGCTCTGCAAT-3) reverse primers and 18S (5'-TTCAAAGTAAACGTGTCGGC-3) sequential PCR sequencing. Sequencing results were analyzed using MEGA 6 software for phylogenetic tree construction using the Neighbor-Joining and Maximum Parsimony Methods. The results showed that the sample Haematopinus sp. originating from Simmental, Limousin, and PO cattle from Jember, and Simmental and PO from Karanganyar were included in one cluster by Haematopinus quadripertusus. Haematopinus sp. sample from Jember FH cattle had a considerable genetic distance from Haematopinus quadripertusus which was possible because the sequence that could be analyzed was only 236 nt.

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Author Biographies

Aan Awaludin, Politeknik Negeri Jember

Program Studi Produksi Ternak, Jurusan Peternakan, Politeknik Negeri Jember

google scholar id: Mll71UMAAAAJ

sinta id: 6004906

scopus id: 57202426366

Yudhi Ratna Nugraheni, Universitas Gadjah Mada

Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Published

10-07-2020

How to Cite

Awaludin, A., Nugraheni, Y. R., Andriani, M., Hasanah, N., & Prayitno, A. H. (2020). Filogenetik Kutu Penghisap Darah (Haematopinus sp.) pada Beberapa Jenis Sapi berdasarkan Gen 18S rRNA. Jurnal Ilmu Peternakan Terapan, 3(2), 31–37. https://doi.org/10.25047/jipt.v3i2.1918

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