Verifikasi Metode Penetapan Kadar Fenol Total Cara Folin-Ciocalteu
DOI:
https://doi.org/10.25047/plp.v4i2.6193Keywords:
Method verification, total phenol content, extract, ISO/IEC 17025Abstract
The Biofarmaka Laboratory is currently preparing for ISO/IEC 17025 accreditation, which requires the use of validated analytical methods and verified procedures for standard methods. This study aims to support the verification of a method for determining total phenolic content using the Folin–Ciocalteu reagent, as outlined in the Indonesian Herbal Pharmacopoeia, First Edition (2013). Verification was conducted by evaluating linearity, precision, accuracy, limit of detection (LoD), and limit of quantification (LoQ). All parameters met the acceptance criteria based on ICH Q2(R1) and ISO/IEC 17025:2017 guidelines, with a determination coefficient (r²) of 0.9993, precision (%RSD) of 1.67%, and accuracy (% recovery) of 101.40%. The LoD and LoQ values were 7.06 ppm and 21.40 ppm, respectively, indicating the method's sensitivity in detecting and quantifying low concentrations of total phenolics. Although the analysis was conducted on green tea extract (Camellia sinensis), the method is generally applicable to various other plant extracts. These results demonstrate that the method is suitable for routine laboratory use and reliable for implementation in support of ISO/IEC 17025-based quality systems.
Downloads
References
[1] AOAC International. (2016). Guidelines for validation of analytical methods for the AOAC Official Methods of Analysis (20th ed.). AOAC International.
[2] Azwanida, N. N. (2015). A review on the extraction methods used in medicinal plants: Principles, strengths, and limitations. Medicinal & Aromatic Plants, 4(3), 196. https://doi.org/10.4172/2167-0412.1000196
[3] European Commission. (2021). SANTE/11312/2021: Analytical quality control and method validation procedures for pesticide residues analysis in food and feed (Version 1). Publications Office of the European Union. https://food.ec.europa.eu/system/files/2021-12/pesticides_mrl_guidelines_wrkdoc_2019-12682.pdf
[4] Gandjar, I. G., & Rohman, A. (2012). Kimia farmasi analisis. Pustaka Pelajar.
[5] Hadi, A., & Asiah. (2020). Verifikasi metode pengujian air dan air limbah: Mendukung penerapan ISO/IEC 17025:2017. Balai Teknologi Laboratorium Lingkungan – Badan Pengkajian dan Penerapan Teknologi.
[6] Handa, S. S., Khanuja, S. P. S., Longo, G., & Rakesh, D. D. (2008). Extraction technologies for medicinal and aromatic plants (pp. 21–54). ICS-UNIDO.
[7] Handoyo, A., Santoso, B., & Riyadi, S. (2022). Validasi metode analisis kuantitatif pada sediaan farmasi menggunakan spektrofotometri UV-Vis. Indonesian Journal of Pharmaceutical Science and Technology, 11(2), 101–109. https://doi.org/10.24198/ijpst.v11i2.42189
[8] Harmita. (2004). Petunjuk pelaksanaan validasi metode dan cara perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117–135. https://journal.farmasi.ui.ac.id/index.php/jfik/article/view/338 (jika tersedia, link jurnal atau pdf)
[9] International Conference on Harmonisation. (2005). ICH Q2(R1): Validation of analytical procedures: Text and methodology. ICH Expert Working Group. https://database.ich.org/sites/default/files/Q2%28R1%29%20Guideline.pdf
[10] International Organization for Standardization. (2017). ISO/IEC 17025:2017 – General requirements for the competence of testing and calibration laboratories. ISO.
[11] Kementerian Kesehatan Republik Indonesia. (2013). Farmakope Herbal Indonesia (Edisi I). Kementerian Kesehatan RI.
[12] Kementerian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia (Edisi II, Suplemen I). Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan.
[13] Nurwanti, S., Suryani, E., & Sari, D. (2024). Implementasi sistem manajemen mutu ISO/IEC 17025:2017 pada laboratorium pengujian material konstruksi Kabupaten Badung. Jurnal Syntax Admiration, 6(1), 199–207. https://doi.org/10.46799/jsa.v6i1.2024
[14] Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11), 1142–1145. https://www.ajol.info/index.php/ajb/article/view/42883
[15] Saman, M. S., Mulyani, T., & Rahayu, I. (2021). Validasi metode spektrofotometri untuk penetapan flavonoid total ekstrak etanol daun belimbing wuluh. Jurnal Ilmu Kefarmasian Indonesia, 19(1), 67–74. https://doi.org/10.35814/jifi.v19i1.2320
[16] Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
[17] Snyder, L. R., Kirkland, J. J., & Dolan, J. W. (2011). Introduction to modern liquid chromatography (3rd ed.). John Wiley & Sons. https://doi.org/10.1002/9781118022830
[18] Waterhouse, A. L. (2002). Determination of total phenolics. Current Protocols in Food Analytical Chemistry, 1(1), I1.1.1–I1.1.8. https://doi.org/10.1002/0471142913.faf0101s00
[19] Yuwono, M., & Indrayanto, G. (2005). Validation of chromatographic methods of analysis. In H. G. Brittain (Ed.), Profiles of drug substances, excipients and related methodology (Vol. 32, pp. 243–259). Academic Press. https://doi.org/10.1016/S0099-5428(05)32009-0.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Dewi Primayanti Laela
This work is licensed under a Creative Commons Attribution 4.0 International License.
Semua orang diperbolehkan:
- Berbagi — menyalin dan menyebarluaskan kembali materi ini dalam bentuk atau format apapun.
- Adaptasi — menggubah, mengubah, dan membuat turunan dari materi ini untuk kepentingan apapun, termasuk kepentingan komersial.
Berdasarkan ketentuan berikut:
- Attribution — Anda harus mencantumkan nama yang sesuai, mencantumkan tautan terhadap lisensi, dan menyatakan bahwa telah ada perubahan yang dilakukan. Anda dapat melakukan hal ini dengan cara yang sesuai, namun tidak mengisyaratkan bahwa pemberi lisensi mendukung Anda atau penggunaan Anda.
- Tidak ada pembatasan tambahan — Anda tidak dapat menggunakan ketentuan hukum atau sarana kontrol teknologi yang secara hukum membatasi orang lain untuk melakukan hal-hal yang diizinkan lisensi ini.
