Stingless bee honey adding butterfly pea flower kombucha on broiler carcass and cholesterol content
DOI:
https://doi.org/10.25047/jipt.v9i1.6134Keywords:
Butterfly pea flower, Broiler, Cholesterol, Cholesterol, Kombucha, Stingless bee honeyAbstract
This study evaluated the effect of incorporating stingless bee honey (Trigona spp.) into butterfly pea flower (Clitoria ternatea L.) kombucha in drinking water on cholesterol levels, abdominal fat, and carcass yield in broilers. A completely randomized design with five treatments (T0: control; T1-T4: kombucha with 2%, 3%, 4%, and 5% stingless bee honey) and four replicates were used. Treatments were administered through drinking water for two weeks. Results showed that honey-enriched kombucha significantly reduced cholesterol levels, with the best result observed in T4 (95±4.63 mg/100 g). Abdominal fat decreased and carcass percentage increased, although not statistically significant. These findings suggest that stingless bee honey-enriched kombucha has potential as a natural feed additive to produce healthier broiler meat, likely mediated through enhanced antioxidant activity and inhibition of HMG-CoA reductase.
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Abed, H., Sabouni, R., & Ghommem, M. (2024). MOF-based spectrophotometric sensors for cholesterol detection: current trends and challenges. RSC Advances, 14(53), 39472–39497. https://doi.org/10.1039/d4ra07476a
Ajibola, A., Chamunorwa, J. P., & Erlwanger, K. H. (2012). Nutraceutical values of natural honey and its contribution to human health and wealth. Nutrition and Metabolism, 9(61), 1–12. https://doi.org/10.1186/1743-7075-9-61
Ali, M., Lee, S. Y., Park, J. Y., & Nam, K. C. (2021). Evaluation of meat from native chickens: analysis of biochemical components, fatty acids, antioxidant dipeptides, and microstructure at two slaughter ages. Food Science of Animal Resources, 41(5), 788–801. https://doi.org/10.5851/KOSFA.2021.E36
Alkhalifah, M. K., Alabduljabbar, K. A., & Alkhenizan, A. H. (2021). Effect of natural honey on lowering lipid profile. Saudi Medical Journal, 42(5), 473–480. https://doi.org/10.15537/SMJ.2021.42.5.20200664
Biluca, F. C., de Gois, J. S., Schulz, M., Braghini, F., Gonzaga, L. V., Maltez, H. F., & Fett, R. (2017). Phenolic compounds, antioxidant capacity and bioaccessibility of minerals of stingless bee honey (Meliponinae). Journal of Food Composition and Analysis, 63(2017), 89–97. https://doi.org/10.1016/j.jfca.2017.07.039
Chen, J. T., He, P. G., Jiang, J. S., Yang, Y. F., Wang, S. Y., Pan, C. H., & Pan, J. M. (2023). In vivo prediction of abdominal fat and breast muscle in broiler chicken using live body measurements based on machine learning. Poultry Science, 102(102239), 1–10. https://doi.org/10.1016/j.psj.2022.102239
Christian, Y. E., Rahmat, D., & Farida, Y. (2022). Standardization of ethanol extract 96% Cantigi leaves (Vaccinium varingiaefolium Miq.). Jurnal Ilmu Kefarmasian Indonesia, 20(2), 225–231. https://doi.org/10.35814/jifi.v20i2.1255
Ding, X., Giannenas, I., Skoufos, I., Wang, J., & Zhu, W. (2023). The effects of plant extracts on lipid metabolism of chickens — A review. Animal Bioscience, 36(5), 679–691. https://doi.org/10.5713/ab.22.0272
Erwan, E., Wiryawan, I. K. G., Syamsuhaidi, S., Purnamasari, D. K., Sumiati, S., & Muhsinin, M. (2022). Antibacterial and antioxidant activity of honey Trigona Sp. in North Lombok District. TERNAK TROPIKA Journal of Tropical Animal Production, 23(1), 18–28. https://doi.org/10.21776/ub.jtapro.2022.023.01.3
Esa, N. E. F., Mohamed, N. M. A., Saiful, I. A. R., Norjihada, I. I., Norhana, J., Nurliyana, A. Z., & Nadirul, H. M. N. (2022). A review on recent progress of stingless bee honey and its hydrogel-based compound for wound care management. Molecules, 27(3080), 1–20. https://doi.org/https://doi.org/10.3390/molecules27103080
Fakolade, P. O. (2015). Effect of age on physico-chemical, cholesterol and proximate composition of chicken and quail meat. African Journal of Food Science, 9(4), 182–186. https://doi.org/10.5897/ajfs2015.1282
Fouad, A. M., & El-Senousey, H. K. (2014). Nutritional factors affecting abdominal fat deposition in poultry: a review. Asian-Australasian Journal of Animal Sciences, 27(7), 1057–1068. https://doi.org/10.5713/ajas.2013.13702
Giampietro-Ganeco, A., Boiago, M. M., Mello, J. L. M., DE SOUZA, R. A., Ferrari, F. B., DE SOUZA, P. A., & Borba, H. (2020). Lipid assessment, cholesterol and fatty acid profile of meat from broilers raised in four different rearing systems. Anais Da Academia Brasileira de Ciencias, 92, 1–14. https://doi.org/10.1590/0001-3765202020190649
Guo, J., Qu, L., Shao, D., Wang, Q., Li, Y., Dou, T., & Tong, H. (2024). Genetic architecture of abdominal fat deposition revealed by a genome-wide association study in the laying chicken. Genes, 15(1), 1–12. https://doi.org/10.3390/genes15010010
Halder, N., Sunder, J., De, A. K., Bhattacharya, D., & Joardar, S. N. (2024). Probiotics in poultry: a comprehensive review. The Journal of Basic and Applied Zoology, 85(23), 1–17. https://doi.org/10.1186/s41936-024-00379-5
Ivanišová, E., Meňhartová, K., Terentjeva, M., Harangozo, Ľ., Kántor, A., & Kačániová, M. (2020). The evaluation of chemical, antioxidant, antimicrobial and sensory properties of kombucha tea beverage. Journal of Food Science and Technology, 57(5), 1840–1846. https://doi.org/10.1007/s13197-019-04217-3
Jalal, M. A. R., Zakaria, H. A. H., Hayajneh, F. M., & Mehyar, G. M. (2023). Performance, carcass characteristics, and meat quality of broiler chickens fed β-mannanase and two levels of energy. Tropical Animal Science Journal, 46(2), 190–200. https://doi.org/10.5398/tasj.2023.46.2.190
Keng, C. B., Haron, H., Talib, R. A., & Subramaniam, P. (2017). Physical properties, antioxidant content and anti-oxidative activities of Malaysian Stingless Kelulut (Trigona spp.) honey. Journal of Agricultural Science, 9(13), 32–40. https://doi.org/10.5539/jas.v9n13p32
Kushargina, R., Rimbawan, R., Dewi, M., Damayanthi, E., & Utami, I. N. (2025). Potential of Butterfly pea flower (Clitoria ternatea L.) tea and kombucha as neutracetical drinks to improve lipid profile of dyslipidemia subjects. Journal of Functional Food and Nutraceutical, 6(2), 94–105. https://doi.org/10.33555/jffn.v6i2.5
Maneesai, P., Iampanichakul, M., Chaihongsa, N., Poasakate, A., Potue, P., Rattanakanokchai, S., & Pakdeechote, P. (2021). Butterfly pea flower (Clitoria ternatea Linn.) extract ameliorates cardiovascular dysfunction and oxidative stress in nitric oxide-deficient hypertensive rats. Antioxidants, 10(523), 1–16. https://doi.org/10.3390/antiox10040523
Milićević, D., Vranić, D., Mašić, Z., Parunović, N., Trbović, D., Nedeljković-Trailović, J., & Petrović, Z. (2014). The role of total fats, saturated/unsaturated fatty acids and cholesterol content in chicken meat as cardiovascular risk factors. Lipids in Health and Disease, 13(41), 1–12. https://doi.org/10.1186/1476-511X-13-42
Palma, M., Huertas, J. R., & Pérez, C. (2008). A comprehensive review of the effect of honey on human health. Nutrients 2023, 15(3056), 1–26. https://doi.org/10.1002/9783527619658.ch32
Permatasari, H. K., Nurkolis, F., Gunawan, W. Ben, Yusuf, V. M., Yusuf, M., Kusuma, R. J., & Tsopmo, A. (2022). Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha. Current Research in Food Science, 5(2022), 1251–1265. https://doi.org/10.1016/j.crfs.2022.08.005
Qaid, M. M., Al-Mufarrej, S. I., Azzam, M. M., & Al-Garadi, M. A. (2021). Anticoccidial effectivity of a traditional medicinal plant, Cinnamomum verum, in broiler chickens infected with Eimeria tenella. Poultry Science, 100(3), 1–9. https://doi.org/10.1016/j.psj.2020.11.071
Rahmawati, O. M., Sugiharto, S., Yudiarti, T., Widiastuti, E., Wahyuni, H. I., Sartono, T. A., & Ayasan, T. (2023). Effect of unripe banana flour as a functional feed ingredient on growth performance, internal organ relative weight and carcass traits of broilers. Veterinary Medicine and Science, 9(2), 851–859. https://doi.org/10.1002/vms3.1070
Ramli, N. Z., Chin, K. Y., Zarkasi, K. A., & Ahmad, F. (2019). The beneficial effects of Stingless bee honey from Heterotrigona itama against metabolic changes in rats fed with high-carbohydrate and high-fat diet. International Journal of Environmental Research and Public Health, 16(4987), 1–17. https://doi.org/10.3390/ijerph16244987
Selvaraj, S., & Gurumurthy, K. (2023). An overview of probiotic health booster-kombucha tea. Chinese Herbal Medicines, 15(2023), 27–32. https://doi.org/10.1016/j.chmed.2022.06.010
Sjofjan, O., Adli, D. N., Natsir, M. H., Nuningtyas, Y. F., Bastomi, I., & Amalia, F. R. (2021). The effect of increasing levels of palm kernel meal containing α-β-mannanase replacing maize to growing-finishing hybrid duck on growth performance, nutrient digestibility, carcass trait, and VFA. Journal of the Indonesian Tropical Animal Agriculture, 46(1), 29–39. https://doi.org/10.14710/JITAA.46.1.29-39
Su, J., Tan, Q., Tang, Q., Tong, Z., & Yang, M. (2023). Research progress on alternative kombucha substrate transformation and the resulting active components. Frontiers in Microbiology, 14, 1–17. https://doi.org/10.3389/fmicb.2023.1254014
Tan, A. F., Ho, A. L., & Chye, F. Y. (2024). Stingless bee honey as functional food-A review. Transactions on Science and Technology, 11(2), 1–13.
Wahyanto, K. N., & Agustini, R. (2024). Total flavonoid content and in vitro anti-inflammatory potentials of kombucha with enrichment of Butterfly pea (Clitoria ternatea) flower extract. Jurnal Pijar Mipa, 19(2), 254–259. https://doi.org/10.29303/jpm.v19i2.6320
Yang, Z. W., Ji, B. P., Zhou, F., Li, B., Luo, Y., Yang, L., & Li, T. (2009). Hypocholesterolaemic and antioxidant effects of kombucha tea in high-cholesterol fed mice. Journal of the Science of Food and Agriculture, 89(1), 150–156. https://doi.org/10.1002/jsfa.3422
Zakaria, Z., Othman, Z. A., Suleiman, J. B., Mustaffa, K. M. F., Jalil, N. A. C., Ghazali, W. S. W., & Kamaruzaman, K. A. (2022). Therapeutic effects of Heterotrigona itama (Stingless bee) bee bread in improving hepatic lipid metabolism through the activation of the keap1/Nrf2 signaling pathway in an obese rat model. Antioxidants, 11(11), 1–21. https://doi.org/10.3390/antiox11112190
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