Identifikasi Genetik Hasil Grafting Tanaman Durian (Durio zibethinus Murr.) Lokal Menggunakan 8 Primer
DOI:
https://doi.org/10.25047/jii.v21i2.2622Keywords:
perbanyakan okulasi, okulasi durian, primer durian, DNA durianAbstract
Grafting merupakan teknik perbanyakan vegetatif yang digunakan untuk mempertahankan karakter unggul durian dari batang atas dan batang bawah. Identifikasi genetik merupakan faktor utama untuk mengetahui apakah tanaman hasil perbanyakan okulasi mempunyai karakter utama dari tanaman induk. Penelitian ini bertujuan untuk mengumpulkan informasi tentang keragaman genetik semai durian dari metode okulasi dan mendapatkan informasi primer terbaik yang dapat digunakan untuk mengidentifikasi genetika durian. Penelitian ini dilakukan di Laboratorium Bioteknologi Fakultas Pertanian Universitas Brawijaya, Malang. Delapan primer yang digunakan adalah OPAI-15, OPB-08, OPAB-14, OPF-13, OPB-10, OPC-05, OPH-15, OPC-08 untuk mengidentifikasi 6 sampel (2 induk untuk batang atas dan batang bawah) dan 4 sampel. sampel dari okulasi bibit. Hasil penelitian menunjukkan dari 6 sampel dan 8 primer yang digunakan untuk identifikasi, sebagian besar sampel hasil perbanyakan cangkok menunjukkan karakteristik batang bawah daripada karakteristik batang bawah. Hasil penelitian juga menunjukkan bahwa 8 primer dari penelitian ini dapat digunakan untuk identifikasi DNA tanaman durian.
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