Studi Molecular Docking Senyawa Peptida Kulit Melinjo (Gnetum Gnemon L.) terhadap Protein Keap1 untuk Aktivasi Sistem Antioksidan
Keywords:
antioksidan, keap1, kulit melinjo, LC HRMS, peptidaAbstract
Stres oksidatif merupakan suatu kondisi yang tidak seimbang antara produksi radikal bebas dan metabolit reaktif. Ketidakseimbangan ini menyebabkan kerusakan pada sel. Eliminasi radikal bebas dapat dilakukan melalui mekanisme perlindungan dengan antioksidan. Keap1 sebagai regulator negatif utama jalur Nrf2 yang berperan dalam pengaturan respons antioksidan endogen. Interaksi antara Keap1 dan Nrf2 pada kondisi basal menyebabkan degradasi Nrf2. Senyawa bioaktif yang mampu menghambat interaksi Keap1-Nrf2 berpotensi meningkatkan aktivasi Nrf2 sehingga akan menginduksi ekspresi berbagai enzim antioksidan endogen. Penelitian sebelumnya melaporkan senyawa peptida juga mampu meningkatkan ekspresi berbagai enzim antioksidan. Pada penelitian ini bertujuan melakukan profiling peptida dari kulit melinjo menggunakan LC-HRMS dan memprediksi kemampuan peptida dalam mengikat Kelch-like ECH-associated protein 1 (keap1) melalui molecular docking. Hasil molecular docking membuktikan bahwa senyawa peptida yang terindentifikasi mampu mengikat protein keap1 dengan terbentuknya ikatan hidrogen dan interaksi hidrofobik. Senyawa peptida L-Leucyl-L-asparaginyl-L-tyrosine (LNY); Ile-trp (IW); Leu-gly-phe (LGF); dan Val-tyr-val (VYV) mempunyai nilai binding affinity yang tinggi, masing-masing -8,6; -8,3; -8,2 dan -8,1 kkal/ mol.
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