Viabilitas Sel dan Aktivitas Antimikroba Bio-Kapsul Probiotik Lb paracasei ssp paracasei ML3 Hasil Ekstrusi Karagenan-SKIM
DOI:
https://doi.org/10.25047/jii.v20i3.2355Keywords:
Antimicrobial Activity, Bio-Capsules, Carregeenan-Skim, Probiotik, Viabilitas SelAbstract
Pembuatan biokapsul Lactobacillus paracasei ssp paracasei Ml3 menggunakan coating agent karagenan dan susu skim sebagai pelindung dan penstabil kelangsungan hidup sel bakteri yang dienkapsulasi selama proses. Penelitian ini dilakukan untuk menguji viabilitas Lactobacillus paracasei ssp paracasei Ml3 dan aktivitas antimikroba terhadap Eschericia coli pada biokapsul basah dan kering setelah kontak 3 jam. Pembuatan biokapsul menggunakan metode ekstrusi, dengan perbandingan bahan pelapis karagenan dan susu skim yaitu 1:1, 2:1, dan 3:1. Â Setiap perlakuan diulang 3 kali. Hasil penelitian menunjukkan bahwa bahan penyalut (karagenan: susu skim) dengan perbandingan 2:1 pada biokapsul basah dan kering mampu mempertahankan viabilitas Lactobacillus paracasei ssp paracasei Ml3 dan hanya menurunkan 0,72 log cfu/g dan 1,31 log cfu/ g, masing-masing. Aktivitas antimikroba ditunjukkan dengan penurunan jumlah Eschericia coli tertinggi setelah kontak 3 jam dengan biokapsul 2:1 yaitu masing-masing 2,21 log cfu/g dan 2,15 log cfu/g.
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Copyright (c) 2020 Ir. Mutia Elida M.Si, Gusmalini Gusmalini, Agustina Agustina, Iza Ayu Saufani
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